Table 1 Primers designed in the current study for amplification of cDNA of leukemic cells K562
Gene | Gene description | Primers (5’ → 3’) |
|---|---|---|
β-actin | beta-actin (housekeeping gene) | F: ACCAACTGGGACGACATGGAG R: GTGAGGATCTTCATGAGGTAGTC |
MYC | c-myc proto-oncogene product, recruits histone acetyltransferases | F: CTCTCAACGACAGCAGCCCG R: CCAGTCTCAGACCTAGTGGA |
RELA | RELA proto-oncogene (p65), NF-kB subunit | F: AGGCTATCAGTCAGCGCATC R: TCCCCACGCTGCTCTTCTAT |
JUN | JUN proto-oncogene, AP-1 transcription factor subunit | F: GAGCTGGAGCGCCTGATAAT R: CCCTCCTGCTCATCTGTCAC |
FOS | forms a heterodimer with JUN | F: GCTGGCGTTGTGAAGACCAT R: TTGGTCTGTCTCCGCTTGGA |
BAX | BCL-2 associated X-protein | F: TCAGGATGCGTCCACCAAGAAG R: TGTGTCCACGGCGGCAATCATC |
CAS9 | caspase 9 (initiator caspase) | F: CGAACTAACAGGCAAGCAGC R: GTCTTTCTGCTCGACATCACC |
CDK4 | Cyclin-dependent kinase 14 | F: GTGTATGGGGCCGTAGGAAC R: CCATAGGCACCGACACCAAAT |
MAPK14 | member of the p38 mitogen-activated protein kinase (MAPK) family | F: TAACAGGATGCCAAGCCATGAG R: GCTTGGGCCGCTGTAATTC |
BCL-2 | B-cell lymphoma 2 | F: ATACCATGATAGCGCCCTTG R: AATCACAGCGAACCTCTGCT |
TNFα | tumor necrosis factor-alpha | F: CTCTTCTGCCTGCTGCACTTTG R: ATGGGCTACAGGCTTGTCACTC |
ABL1 | Philadelphia translocation | F: GACATGCCATAGGTAGCAATTTCCC R: ACATCACGCCAGTCAACAGTCTGG |