Table 1 Primer sequences used in this study
Human genes | Primer sequence | Length (bp) |
|---|---|---|
Collagen type I alpha 1 chain (COL1A1) | Forward: 5′-TCAAGAGAAGGCTCACGATGG-3′ Reverse: 5′-TCACGGTCACGAACCACATT-3′ | 69 |
Collagen type I alpha 2 chain (COL1A2), | Forward: 5′-GGTCAGCACCACCGATGTC-3′ Reverse: 5′- CACGCCTGCCCTTCCTT-3′ | 51 |
Collagen type III alpha 1 chain (COL3A1), | Forward: 5′- GCTGGCTACTTCTCGCTCTG-3′ Reverse: 5′- TCCGCATAGGACTGACCAAG-3′ | 97 |
Cellular communication network factor 2 (CCN2) | Forward: 5′- CAGCATGGACGTTCGTCTG-3′ Reverse: 5′- AACCACGGTTTGGTCCTTGG-3′ | 115 |
Fibronectin 1 (FN1) | Forward: 5′- TTCTAAGATTTGGTTTGGGATCAAT-3′ Reverse: 5′- TCTTGGTTGGCTGCATATGC-3′ | 51 |
Transforming growth factor beta 1 (TGFB1) | Forward: 5′- CAATTCCTGGCGATACCTCAG-3′ Reverse: 5′- GCACAACTCCGGTGACATCAA-3′ | 86 |
Smooth muscle actin alpha 2 (ACTA2) | Forward: 5′- GACAATGGCTCTGGGCTCTGTAA-3′ Reverse: 5′-CTGTGCTTCGTCACCCACGTA-3′ | 147 |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | Forward: 5′-GACCACTTTGTCAAGCTCATTTCC-3′ Reverse: 5′-GTGAGGGTCTCTCTCTTCCTCTTGT-3′ | 151 |