Fig. 3
Immunohistochemical staining: A Normal hepatocytes showing a weak immunoreactivity against TNF α. B CCl4 treated groups, showing strongest immunoreactivity in Kupffer cells and endothelium of the central vein. C CR treated group, is showing lesser immunoreactivity. D Staining quantification of TNF α using image j software. E Normal hepatocyte is showing a weak immunoreactivity against Arginase I. F CCl4 treated group, the cytoplasm of the hepatocytes (red squares) exhibiting strong reactivity within the hepatocyte’s cytoplasm and Kupffer cells (short arrows). G: The hepatocytes show lesser immunoreactivity in CR ethyl acetate extract. H Staining quantification of Arginase I, using image j software. I Increasing in the number of the Bcl-2 positive hepatocytes (arrows). J CCl4 treated group, is showing decrease in Bcl-2 positive cells. K: CR ethyl acetate extract treated groups. L Staining quantification of Bcl-2 using image j software. M Normal group, the cytoplasm of the periportal hepatocytes is showing a staining against PPAR α (arrows). N CCl4 treated group, is showing an increase in PPAR α positive cells. O CR ethyl acetate extract treated group. P Staining quantification of PPAR α using image j software. The CR extract treated groups is compared to control and CCl4 groups using Tukey test (n = 3). *P ≤ 0.05