Fig. 4

Effect of 8-MNA and capsaicinoids on AMPK activity in 3T3-L1 adipocytes. Western blotting (a) and quantification (b-d) of cell lysates collected from 3T3-L1 adipocytes cultured with vehicle (Veh), 8-MNA (1 and 10 μM), CAP (1 μM) or DHC (1 μM) during 48 h starvation. Data are presented as mean ± SEM and analyzed by one-way ANOVA in (b) (n = 4) and by Student’s t-test in (c and d) (n = 4–5). * p < 0.05 vs. Veh. n, sample size. The original blots are presented in Supplementary Figure