Fig. 3

Effect of 8-MNA and capsaicinoids on fat accumulation in 3T3-L1 adipocytes. Representative images (a-f) and quantification (g-i) of ORO staining in 3T3-L1 adipocytes cultured with vehicle (Veh), 8-MNA (1 μM), CAP (1 μM), or DHC (1 μM) during 48 h starvation. Bright-field images were taken at 20X magnification. White arrows indicate representative lipid droplets. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed by Student’s t-test (n = 4–6). * p < 0.05 vs. Veh. n, sample size