Fig. 2

Effect of Ziziphus honey and its isolated protein treatment on ROS production (a) Treatment with low doses (3.12–100 μg/mL) of honey (b) Treatment with high doses (3.12–100 mg/mL) of honey (c) Treatment of isolated honey proteins (0.4—400 ng/mL). Results are presented in relative light units (RLU) and oxidative burst activity of whole blood using luminol as a probe. Each vertical bar represents a mean of triplicate. Error bars represent standard deviation (SD) of the means. Significance difference was compared to the control (C) where C = Cells stimulated with zymosan and *** represents the P-value of ≤ 0.001, ** (P ≤ 0.01), and * (P ≤ 0.05). Statistical analysis was done by using one-way ANOVA