Table 1 The product and manufacturers used in this investigation

RPMI1640 media

Gibco BRL Co.

Fetal bovine serum (FBS)

Penicillin-streptomycin

Phosphate-buffered saline (PBS)

Trypsin-EDTA (TE)

Lipopolysaccharide (LPS, O55:B5)

Sigma-Aldrich

Dimethyl sulfoxide (DMSO)

3-(4,5-Dimethylathiazol-2yl)-2,5-diphenyltetrazoleum (MTT)

Ammonium persulfate (APS)

Tween-20

PGE₂ ELISA kit

ENZO Life Science, Inc.

IL-6 ELISA kit

R&D System

TNF-α ELISA kit

RIPA lysis buffer

Thermo Fisher Scientific

Protease and phosphatase inhibitor cocktail

NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Nitrocellulose (NC) membrane

Bio-Rad Laboratories, Inc.

Bis-acrylamide solution

Tris-HCl

Tetramethylethylenediamine (TEMED)

Tris-glycine SDS buffer

GenDepot

Tris-glycine native buffer

Sodium dodecyl sulfate (SDS)

Tris-buffered saline (TBS)

ECL solution

Skimmed milk powder

BD Biosciences

Anti-iNOS

Cell Signaling Technology Inc.

Anti-COX-2

Anti-IкB-α

Anti-p-IкB-α

Anti-p65

Anti-p-ERK

Anti-ERK

Anti-p-JNK

Anti-JNK

Anti-p-p38

Anti-p38

Anti-HO-1

Anti-Nrf2

Anti-β-actin

Santa Cruz Biotechnology Inc.

Anti-PCNA

Secondary antibodies

Merck Millipore Co.