Fig. 7

The inhibitory effect of SnPP on CLE-mediated reduction of NO, PGE₂, iNOS, and COX-2. Cells were pre-treated with 150 µg/mL of CLE for 3 h with or without pre-treatment with SnPP for 1 h, and then stimulated with LPS (1 µg/mL) for 24 h. A, B The concentration of NO and PGE₂ was determined by Griess assay and ELISA, respectively. Data represent the mean values of three independent experiments ± SD. ***p < 0.001. C The levels of iNOS and COX-2 proteins were determined by Western blot analysis. β-Actin was used as a loading control. Representative blots from three independent experiments are shown