Fig. 5

The effect of CLE on the activation of MAPK signaling pathway in LPS-induced BV2 microglial cells. Cells were pre-treated with indicated concentrations of CLE for 3 h, and then stimulated with LPS (1 µg/mL) for 1 h. The phosphorylated and total forms of p38, ERK, and JNK were determined by Western blot analysis. β-Actin and was used as a loading control. Representative blots from three independent experiments are shown