Fig. 4

The effect of CLE on the activation of NF-κB signaling pathway in LPS-induced BV2 microglial cells. Cells were pre-treated with indicated concentrations of CLE for 3 h, and then stimulated with LPS (1 µg/mL) for 1 h. Nuclear and cytosolic extracts were isolated and the levels of p-IκB-α and IκB-α in the cytosolic fraction, and p65 in the nuclear faction were determined by Western blot analysis. β-Actin and PCNA were used as a loading control for cytoplasm and nuclear, respectively. Representative blots from three independent experiments are shown