Fig. 2

The effect of CLE on the production of NO and PGE₂ (A, B), and the overexpression of iNOS and COX-2 (C) in LPS-induced BV2 microglial cells. Cells were pretreated with/without the indicated concentrations of CLE for 3 h and them stimulated with LPS (1 µg/mL) for 24 h. A, B The concentration of NO and PGE₂ was determined by Griess assay and ELISA, respectively. Data represent the mean values of three independent experiments ± SD. ^#p < 0.001 compared to the control group; **p < 0.01, and ***p < 0.001 compared to the LPS treated group. C The levels of iNOS and COX-2 proteins were determined by Western blot analysis. β-Actin was used as a loading control. Representative blots from three independent experiments are shown