Fig. 4

Effects of TJ-41 on tail-suspended mice. A Mice were subjected to 7 days of pretreatment and 24 h of tail-suspension. Effect of TJ-41 treatment on the expression of atrogin-1 and MuRF1 in gastrocnemius muscles of tail-suspended mice (n value is 10, 10, 10, 9, 10, 9 in each group, respectively, and total number is 58). B–D Mice were subjected to pretreatment for 7 days and tail-suspension for 14 days. The ratios of body weight changes during experiments (B), average food intake per day (C), and effects of TJ-41 treatment on the weight of gastrocnemius muscles (D). The low-dose and high-dose groups received 0.3 and 1.0 g TJ-41 per kg body weight, respectively (n value is 9, 10, 10, 9, 10, 8 in each group, respectively, and total number is 56). *p < 0.05, **p < 0.01, ***p < 0.001. NS indicates “not significant.”