Fig. 6

Histological changes in Q- BDL group: The hematoxylin and eosin-stained sections: 6A. Q-BDL group shows relatively thin interalveolar septa (↑) with patent alveoli (A). Bronchiole (B) appears with intact epithelial lining (^). Notice a relatively thin walled less congested and less dilated pulmonary blood vessel (V). (× 100). 6 B. Q-BDL shows apparent increase in number of pneumocyte type I (I). Few mononuclear cellular infiltrations (*) are apparent within some relatively thin interalveolar septa (↑), (II) = pneumocyte type II, (A) = alveoli (× 400). Toluidine Blue stained semi thin sections: 6C. Q-BDL group shows relatively thin interalveolar septa with less mononuclear cellular infiltration. Notice the mild congestion of few blood vessels(C) with apparent increase in number of thin type- I pneumocyte with their flat nuclei (I), (II) = pneumocyte type II. (× 1000). 6D. In Q-BDL many of the terminal bronchiole are similar to the control, with its epithelial lining showed two types of cells: ciliated cells (arrow) and non-ciliated cells (Clara cells) (arrowhead). Clara cell has dome shaped apex protruding into the lumen and has narrow base. It had more darkly stained oval indented nucleus, slips of smooth muscle (M) surround the bronchiole also can be seen (× 1000). Photomicrographs of Masson’s trichrome stained sections of rat lung: 6E. The Q-BDL group presents apparent decrease deposition of collagen fibers nearly similar to the sham-operated group in the form of thin fine collagen fibers in the interalveolar septa (↑), per bronchial (B) and perivascular (V) areas, alveoli = (A). (Masson’s trichrome × 100). Photomicrographs of PAS-stained sections of rat lung: 6F. Q-BDL group presents positive PAS reaction (Q-BDL) in bronchiolar passages, wall of a blood vessel, and walls of alveoli. (PAS X 100). 6G. Photomicrographs of Ultrastructure examination by Transmission electron microscope (TEM) of lung: Q-BDL group showing of pneumocyte type-I (PI) with thin attenuated cytoplasm toward the alveolar space and flattened nucleus (n). Dome shaped type-II pneumocyte (PII) and many dense full lamellar bodies (LB) and normal microvilli (red arrow).CF = collagen fibrils (Uranyl acetate and Lead Citrate X120000)