Fig. 4

Histological changes in sham-operated group.The hematoxylin and eosin-stained sections. 4A. Sham group shows normal lung architecture with thin interalveolar septa (↑). Notice patent alveolar sacs (S), alveoli (A), bronchiole, (B) and pulmonary vessels (V). (× 100). 4B. Sham group shows thin interalveolar septa (↑). The bronchiole (B) is lined by simple columnar ciliated epithelium (^) and is surrounded by concentric layers of smooth muscle fibers (M). The alveoli (A) are mostly lined by thin type I pneumocyte with their flat nuclei (I) and few cubical pneumocytes type II with their large, rounded nuclei and vacuolated cytoplasm (II) present at the angles of interalveolar septa and pulmonary vessels (V). (× 400). Toluidine Blue stained semi thin sections: 4C. Sham group shows a terminal bronchiole, its epithelial lining showed two types of cells; ciliated cells (arrow) and non-ciliated cells (Clara cells) (arrowhead), with dome shaped apex protruding into the lumen and has narrow base. It had more darkly stained oval indented nucleus, slips of smooth muscle (M) are seen surrounded the bronchiole also can be seen. (× 1000). 4D. Sham group shows interalveolar septa containing capillaries and few inflammatory cells separating wide air spaces. The alveoli seen lined by mostly with flat pneumocyte type I (I) and few cubical pneumocytes type II with their large, rounded nuclei and vacuolated cytoplasm (II) present at the angles of interalveolar septa. (× 1000). 4E. Masson’s trichrome stained lung sections: Sham group has few green collagen fibers (↑) surrounding mainly the walls of the blood vessels (V). Notice scanty collagen fibers present in the alveolar septa (↑) and around the bronchiole (B). A = alveoli. (x100) 4F. The sham-operated group shows strong positive PAS reaction. In Photomicrographs of Ultrastructure examination by Transmission electron microscope (TEM) of lung: G. Transmission electron micrographs of the sham group showing patent alveolus (A) showing type-I of pneumocyte type-I (PI) with thin attenuated cytoplasm toward the alveolar space and flattened nucleus (n). dome shaped type-II pneumocyte (PII) with large euchromatic nucleus (N) and many lamellar bodies (blue arrow). (Uranyl acetate and Lead citrate × 8000). 4H. showing type-I of pneumocytetype-I (PI) with thin attenuated cytoplasm toward the alveolar space and flattened nucleus (n). Dome shaped type-II pneumocyte (PII) with large euchromatic nucleus (N). (Uranyl acetate and Lead Citrate × 8000)