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Table 4 The results of matrix effects, recovery, precision, accuracy and stability of six ingredients in plasma (n = 6)

From: Pharmacokinetics and pharmacodynamics of bioactive compounds in Penyanqing preparation in THP-1 inflammatory cells induced by Lipopolysaccharide

Compounds

Concentration

(ng/mL)

matrix effects

(%)

Recovery

(%)

Precision (RSD%)

Accuracy (%)

Room temperature 24 h

Three freeze- thaw cycles

In 56 ℃ for 1 h

Intra-day

Inter-day

Intra-day

Inter-day

measured value

measured value

measured value

 

100

96.55 ± 2.09

95.92 ± 2.07

5.85 ± 5.78

5.19 ± 5.18

98.73 ± 5.78

99.76 ± 5.18

99.68 ± 2.31

97.89 ± 4.06

98.46 ± 2.77

AST

200

102.99 ± 2.75

92.76 ± 2.47

2.85 ± 2.71

3.98 ± 3.87

95.05 ± 2.71

97.36 ± 3.87

199.01 ± 3.99

193.37 ± 7.61

194.85 ± 6.53

 

500

100.24 ± 2.28

94.96 ± 2.16

2.86 ± 2.82

2.43 ± 2.43

98.93 ± 2.82

99.96 ± 2.43

498.07 ± 3.95

497.15 ± 6.61

495.21 ± 8.43

 

50

107.61 ± 2.45

89.83 ± 2.04

7.89 ± 8.08

6.33 ± 6.35

102.43 ± 8.08

100.4 ± 6.35

49.14 ± 2.35

49.41 ± 1.85

48.33 ± 2.76

FE

125

93.48 ± 4.51

90.13 ± 4.35

2.18 ± 2.19

2.61 ± 2.63

100.37 ± 2.19

100.68 ± 2.63

122.72 ± 2.65

124.36 ± 2.46

123.42 ± 1.76

 

400

88.56 ± 2.09

96.54 ± 2.74

1.45 ± 1.48

1.84 ± 1.88

102.23 ± 1.48

101.92 ± 1.88

403.59 ± 7.84

400.59 ± 6.95

399.13 ± 3.26

 

20

92.25 ± 2.35

95.84 ± 2.44

8.48 ± 8.75

6.01 ± 6.25

103.23 ± 8.7

104 ± 6.25

20.81 ± 0.85

20.62 ± 1.06

19.88 ± 0.43

PRO

100

108.34 ± 1.53

90.61 ± 1.28

6.67 ± 6.62

4.78 ± 4.89

99.17 ± 6.62

102.15 ± 4.89

100.34 ± 1.48

97.77 ± 2.49

98.21 ± 2.33

 

400

105.24 ± 3.06

91.32 ± 2.65

4.3 ± 4.43

3.45 ± 3.48

103 ± 4.43

101.05 ± 3.48

398.27 ± 4.21

395.66 ± 7.14

394.26 ± 8.01

 

100

103.81 ± 3.10

89.57 ± 2.67

4.51 ± 4.36

3.62 ± 3.56

96.56 ± 4.36

98.34 ± 3.56

99.91 ± 2.94

98.55 ± 2.93

97.22 ± 3.31

CH

200

102.10 ± 2.23

95.61 ± 3.02

4.96 ± 4.69

5.13 ± 4.96

94.62 ± 4.69

96.84 ± 4.96

200.03 ± 9.56

191.77 ± 8.91

195.56 ± 7.88

 

500

93.58 ± 1.13

97.97 ± 1.18

6.84 ± 6.75

4.57 ± 4.54

98.66 ± 6.75

99.58 ± 4.54

499.00 ± 6.02

485.90 ± 9.73

489.60 ± 8.91

 

100

102.84 ± 2.17

90.62 ± 1.91

5.87 ± 5.68

4.09 ± 4.03

96.67 ± 5.68

98.7 ± 4.03

99.44 ± 2.43

99.68 ± 2.83

95.32 ± 1.06

PE

200

107.07 ± 0.71

98.69 ± 0.65

1.71 ± 1.75

2.53 ± 2.60

102.79 ± 1.75

102.68 ± 2.60

199.64 ± 2.59

197.55 ± 3.60

196.25 ± 4.08

 

500

100.02 ± 6.36

94.55 ± 6.01

1.83 ± 1.84

2.6 ± 2.62

100.82 ± 1.84

100.71 ± 2.62

499.23 ± 2.58

492.85 ± 7.60

495.87 ± 6.50

 

200

91.94 ± 3.62

97.90 ± 3.85

3.83 ± 3.69

5.12 ± 5.10

96.23 ± 3.69

99.64 ± 5.10

208.88 ± 8.46

194.10 ± 8.56

196.22 ± 3.40

SAB

400

101.21 ± 1.16

92.44 ± 1.06

1.13 ± 1.03

3.2 ± 2.95

90.92 ± 1.03

92.09 ± 2.95

391.06 ± 6.16

388.99 ± 6.75

395.20 ± 10.70

 

800

102.70 ± 1.34

99.81 ± 1.30

3.57 ± 3.39

3.2 ± 2.97

94.98 ± 3.39

92.82 ± 2.97

792.27 ± 3.94

790.71 ± 3.52

791.11 ± 5.98