Fig. 2

Effects of PREO on the production of NO, ROS and MDA and the expression of iNOS at the mRNA and protein levels. PREO significantly lowered the production of oxidative stress mediators in LPS-induced RAW 264.7 cells. A: The content of NO in cell culture medium; B: mRNA expression of iNOS was detected by RT‒PCR; C: Western blot result of iNOS protein; D: The intracellular reactive ROS level; E: Level of MDA in the cell; F: Western blot bands of iNOS.: Band pictures have been cropped (see supplementary data for uncropped version). Values represent means ± SDs (n = 3). Statistical analysis was performed by one-way ANOVA with a Waller-Duncan test. a-f Superscript with different letters denotes significant differences (p < 0.05) between LPS and each sample in pairs