Fig. 5

FJE and PVE inhibits cytokine-induced inflammation through attenuating AKT and NF-κB phosphorylation in ARPE-19 cells. ARPE-19 cells were pretreated with 5 ng/ml of IL-6 + TNF-α cytokines for 2 h and then administered FJE and PVE (30 ng/ml) and incubated for 10 min. IL-6 + TNF-α facilitated an increase in AKT and NF-κB phosphorylation levels in treated ARPE-19 cells compared to control cells. β-actin was used as a reference control. Full length blots are presented in Supplementary Fig. S2. Quantification of protein expression levels by normalization to the internal control, β-actin. Depicted western blots are one representative figure of three independent experiments. Results are the mean ± S.D. of three independent experiments