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Table 2 Inhibitory effect of extracts from Acalypha communis and Solanum atripicifolium on Nile Red transport in yeast cells overexpressing Mdr1 and Cdr1 transporters

From: Extracts from Argentinian native plants reverse fluconazole resistance in Candida species by inhibiting the efflux transporters Mdr1 and Cdr1

Yeast

Plant species

FIR

Extract concentration (µg/mL)

200

100

50

25

12.5

6.25

Candida albicans 1114 (CaMdr1/CaCdr1)

Acalypha communis

2.36 ± 0.41**

1.69 ± 0.07**

1.24 ± 0.17*

1.07 ± 0.12

  

Solanum atripicifolium

3.68 ± 0.36***

2.82 ± 0.45**

2.11 ± 0.46*

1.27 ± 0.17*

1.12 ± 0.15

 

Candida albicans ATCC 90028

Acalypha communis

0.30 ± 0.02**

     

Solanum atripicifolium

1.12 ± 0.19

     

Saccharomyces cerevisiae AD/CaMDR1

Acalypha communis

1.92 ± 0.23*

1.74 ± 0.07***

1.53 ± 0.10*

1.17 ± 0.16

  

Solanum atripicifolium

1.93 ± 0.16*

1.64 ± 0.19*

1.26 ± 0.05*

1.11 ± 0.07

  

Saccharomyces cerevisiae AD/CaCDR1

Acalypha communis

2.10 ± 0.13*

1.94 ± 0.25*

1.52 ± 0.18*

1.25 ± 0.15*

1.10 ± 0.14*

0.80 ± 0.08

Solanum atripicifolium

2.01 ± 0.12*

1.68 ± 0.14*

1.34 ± 0.06*

1.07 ± 0.07

  

Saccharomyces cerevisiae AD1-8u

Acalypha communis

0.70 ± 0.05*

     

Solanum atripicifolium

0.81 ± 0.09

     

Candida glabrata 109 (CgCdr1)

Acalypha communis

1.54 ± 0.18*

1.35 ± 0.10*

1.23 ± 0.12***

1.26 ± 0.17*

1.16 ± 0.11

 

Solanum atripicifolium

2.11 ± 0.31*

2.15 ± 0.19*

1.39 ± 0.10*

1.10 ± 0.08

  

Candida glabrata ATCC 2001

Acalypha communis

0.30 ± 0.04

     

Solanum atripicifolium

0.62 ± 0.04

     
  1. FIR Fluorescence intensity ratio, MFI mean fluorescence intensity of Nile Red in cells incubated with extract/MFI of Nile Red in cells incubated without extract. Significant differences with respect to the negative control were determined by using a paired one-tailed Student’s t test (***p < 0.001, **p < 0.01, *p < 0.05)