Fig. 7

Low expression of NXO4 attenuated the inhibition of LPS-induced ROS production by baicalin. A NOX4 mRNA expression in HAECs infected with control-siRNA, NOX4-siRNA-1, NOX4-siRNA-2 and NOX4-siRNA-3. All NOX4-siRNAs remarkably downregulated NOX4 mRNA. B Western blotting analysis of the expression of NOX4 after the suppression of NOX4 expression. C The expression of ROS content in HAECs transfected with NOX4-siRNA or control-siRNA after stimulation with or without LPS (1 μg/ml). ROS levels were detected by flow cytometry analysis. Data were shown as mean ± SEM. (n = 3, *P < 0.05, ***P < 0.001)