Fig. 6

Baicalin increased the expression levels of NOX4 and decreased the LPS-induced ROS generation. A Effect of different concentrations of baicalin on viability of HAECs assessed by CCK8. B Baicalin modulated mRNA expression level of NOX4 and eNOS. GAPDH was used as the control. C HAECs were cultured with LPS supplemented with baicalin or not. Cell lysates were then analyzed using Western blotting assay. Each histogram value is the ratio of the scan value for NOX4 divided by the value for β-actin. The values represent mean ± SEM of three independent experiments. D Baicalin inhibited ROS release. HAECs were exposed to baicalin for 12 h and stained with 20 μM DCFDA for 30 min, followed by 1 μg/ml LPS for 6 h. ROS levels were detected by flow cytometry analysis. Data were shown as mean ± SEM. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ***** P < 0.0001)