Fig. 8

Effects of NB on protein levels of PI3K/Akt/mTOR pathway in NSCLC cells (H460). (A and B) NSCLC cells (H460) were treated with 0–10 µM of NB for 6 and 24 h. Western blot analysis was performed to detect the mTOR, p-mTOR, PI3K, p-PI3K, Akt, p-Akt, and c-Myc protein levels. The blots were reprobed with β-actin to confirm an equal loading of each sample. Densitometry was used to calculate the protein expression levels and the values of protein levels were presented as the fold changes relative to inactive form of the proteins. The uncropped blotting bands were presented in Additional file 3 and Additional file 4. Data represent the mean ± SD (n = 3) (* 0.01 ≤ p < 0.05, ** p < 0.01, compared with the untreated control)