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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Novel mechanism of napabucasin, a naturally derived furanonaphthoquinone: apoptosis and autophagy induction in lung cancer cells through direct targeting on Akt/mTOR proteins

Fig. 3

Effects of NB and NB-acid on apoptotic cell death including the alteration of apoptotic related proteins in NSCLC cells (H460 and H292). (A) NSCLC cells (H460 and H292) were seeded and treated with 0–25 µM of NB and NB-acid for 24 h. Apoptotic cells were determined using annexin V-FITC/PI staining with flow cytometry. Percentages of cells at each stage were calculated. (B) NSCLC cells (H460) were seeded and treated with 0–10 µM of NB and NB-acid for 24 h. Western blot analysis was performed to detect the PARP, cleaved PARP, Mcl-1, Bcl-2, and Bax protein levels. The blots were reprobed with β-actin to confirm an equal loading of each sample. Densitometry was used to calculate the protein expression levels and the values of protein levels were presented as the fold changes relative to uncleaved form of the protein or β-actin. The uncropped blotting bands were presented in Additional file 1. Data represent the mean ± SD (n = 3) (* 0.01 ≤ p < 0.05, ** p < 0.01, compared with the untreated control)

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