Fig. 4

Formulation feeding does not alter Relish expression levels in GMR driven -127Q expressing eye discs. Confocal projection images showing larval eye imaginal discs stained with anti-Relish in WT eye imaginal disc (a) or GMR-GAL4 > UAS-127Q larval discs when fed on regular food (c) Guduchi (e) or Madhuyashti (g). Nuclei counterstained with DAPI (blue) (b, d, f, h). Scale bar in section A is 50 μm and is applicable to all sections from a-h. B Graph represents the mean (±SD) fluorescence intensities of Relish (Y-axis) in different feeding regimes, drawn through LSM 510 Meta. The blue, yellow, and green bars represent regular food, Guduchi and Madhuyashti respectively