Fig. 2

Formulation feeding disrupts rhabdomere arrangements, increases polyQ inclusion bodies and axonal connections A Confocal projection images show larval eye imaginal discs immunostained with antibodies against anti-ELAV antibody (green) in WT (a) or GMR-GAL4 > UAS-127Q/CyO-GFP larvae fed on regular food (b) Guduchi (c) or Maduyashti (d) and polyQ (red) in WT (e) or GMR-GAL4 > UAS-127Q/CyO-GFP larvae fed on regular food (f) Guduchi (g) or Madhuyashti (h). Scale bar 50 μm, in section a is applicable to sections a to h. Immunostaining of eye imaginal discs with mab-22c10 antibody showed a regular arrangement of neuronal connections in WT (i), which was disrupted in GMR-GAL4 UAS-127Q/CyO-GFP larvae fed on regular food (j) and increase in disruption in Guduchi (k), or Madhuyashti supplemented food (l). Scale bar in section i, 20 μm is applicable to sections i to l. B Graph represents the mean (±SD) fluorescence intensities of IBs (Y-axis) in different feeding regimes, drawn through LSM 510 Meta. **represents P ≤ 0.001 using Student’s t test. The blue, yellow and green bars represent regular food, Guduchi and Madhuyashti respectively