Fig.2

Prediction of the targets of SAA. A Effect of SAA on the formation of C3/C5 convertase of the complement classical pathway. Different concentrations of SAA were incubated with rat serum and sheep red blood cells for 3 min; after the sheep red blood cells were washed, rat serum containing 0.04 mol/L EDTA was added to initiate hemolysis. The absorbance was measured at 412 nm. Data were presented as mean ± SD (n = 4). B Contents of complement component C3 in the supernatant of classical pathway hemolysis experiment. Different concentrations of SAA were incubated with rat serum and sheep red blood cells for 30 min, centrifuge was performed to obtain the supernatant, and ELISA detection of the C3 content was performed. Data were presented as mean ± SD (n = 3). ***P < 0.001 compared with the serum group, statistical tests used were t-tests. C Map of 10 conjugation sites of SAA and C3b protein. D Conjugation diagram of SAA and C3b protein site 1