Fig. 6

Modulation of EMT markers in cervical cancer cells after MNBE treatment. (A) ZO-1 expression was detected by flow cytometry in HeLa, SiHa, and ME-180 cells after treatment with 10, 20, and 30 µg/ml of MNBE for 24 h. (B) Quantitative real-time PCR analysis of MMP2, FAK, N-cadherin, snail, and E-cadherin after cells were treated with 10, 20, and 30 µg/ml of MNBE for 24 h. Data expressed as the mean ± SEM of the three independent experiments. Vehicle-treated cells were used as control. The significant differences are indicated by *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001 vs vehicle control