Fig. 4
Modulation of pro and anti-apoptotic genes in cervical cancer cells after exposure to MNBE. (A) Active caspase-3 expression was detected by flow cytometry in HeLa, SiHa, and ME-180 cells after treatment with IC25 and IC50 of MNBE for 24 h. (B) Western blot analysis of the expression of NFkB-p65 and p53 in HeLa, SiHa, and ME-180 cells treated with MNBE at indicated dose for 24 h. GAPDH in each sample was employed as a standard. (C) Quantification of bands of NFkB-p65 and p53 was performed using Image J software normalized to GAPDH. (D) Quantitative real-time PCR analysis of Bcl2 and survivin after cells were treated with MNBE for 24 h. Data expressed as the mean ± SEM of the three independent experiments. Vehicle-treated cells were used as control. The significant differences are indicated by *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001 vs vehicle control