Fig. 3

Induction of apoptosis in cervical cancer cells after MNBE treatment. Flow cytometry apoptotic cell analysis of MNBE treated HeLa, SiHa, ME-180, and non-cancerous L2 cells using Annexin V–FITC and PI staining. (A) Apoptotic cell death of HeLa, SiHa, and ME-180 cells after treatment with MNBE at their respective IC₂₅ and IC₅₀ doses for 24 h. (B) and (C) L2 cells were treated with 50, 100, and 145 µg/ml of MNBE for 24 h. Bar diagrams represent the percentage of apoptotic cells calculated by FlowJo software. Data expressed as the mean ± SEM of the three independent experiments. Vehicle-treated cells were used as control. The significant differences from control are indicated by *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001 vs vehicle control