Fig. 5
Administration of nontoxicological levels of WEAOR attenuated the expression of photoaging-related genes. Cytotoxicity of WEAOR on NIH-3T3 cells (A). NIH-3T3 cells were treated with various concentrations of WEAOR (0–500 μg) and incubated for 24 h at 37 °C in a CO2 incubator. UVB-mediated expression of COL1A1 and MMP-1a (B), and IL-6, IL-8, and MCP-3 (C) were significantly restored by WEAOR treatment. The relative expression of each gene was estimated using qRT-PCR and normalized by that of GAPDH. Experiments were conducted in triplicate and repeated three times with similar results. Each data value is expressed as mean ± SD. * indicates a significant difference compared with the control (nontreated). Different letters indicate significant differences between groups (P < 0.05)