BMC Complementary Medicine and Therapies

Table 5 Primer sequences designed for RT-PCR (5′-3′)

From: Effects of plant-based medicinal food on postoperative recurrence and lung metastasis of gastric cancer regulated by Wnt/β-catenin-EMT signaling pathway and VEGF-C/D-VEGFR-3 cascade in a mouse model

Gene	Sequences (5'-3')
β-catenin	Forward: GGTGGACCCCAAGCCTTAGTA
β-catenin	Reverse: AGATGAAGCCCCAGTGCCT
GSK-3β	Forward: CCACCATCCTTATCCCTCCA
GSK-3β	Reverse: AGCGGCGTTATTGGTCTGTC
E-cadherin	Forward: GGCTGGACCGAGAGAGTTACC
E-cadherin	Reverse: TGACCTCATTCTCAGGCACTTG
Vimentin	Forward: CCTCTATTCCTCATCCCCCG
Vimentin	Reverse: AACTCAGTGTTGATGGCGTCG
VEGF-C	Forward: TGTGGGGAAGGAGTTTGGAG
VEGF-C	Reverse: CGGCAGGAAGTGTGATTGG
VEGF-D	Forward: TGCCCGAGTTAGTGCCTGTTA
VEGF-D	Reverse: CTTCTTCTGGGGTCTGAATGGA
VEGFR-2	Forward: GATTTTCTCCATCCCCCCA
VEGFR-2	Reverse: GATACTGTCACCACCGCCG
VEGFR-3	Forward: GCCAAGGGCTGCGTAAACT
VEGFR-3	Reverse: CCCAGAAGAAAACTGCGATGA
GAPDH	Forward: CCTTCCGTGTTCCTACCCC
GAPDH	Reverse: GCCCAAGATGCCCTTCAGT

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ISSN: 2662-7671

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