Table 1 Primer sequences used in a quantitative real-time PCR
From: NK cell-mediated immunostimulatory effects of ethanol extract of Morinda citrifolia (noni) fruit
Gene | Direction | Sequences (5’ to 3’) |
|---|---|---|
IL-6 | Forward Reverse | GAC AAA GCC AGA GTC CTT CAG AGA CTA GGT TTG CCG AGT AGA TCT C |
IFN-β | Forward Reverse | AAG AGT TAC ACT GCC TTT GCC ATC CAC TGT CTG CTG GTG GAG TTC ATC |
TNF-α | Forward Reverse | TGC CTA TGT CTC AGC CTC TT GAG GCC ATT TGG GAA CTT CT |
IL-1β | Forward Reverse | GTG AAA TGC CAC CTT TTG ACA GTG CCT GCC TGA AGC TCT TGT TG |
IL-12b | Forward Reverse | TGG AGC ACT CCC CAT TCC TA GAG CTT GCA CGC AGA CAT TC |
IFN-γ | Forward Reverse | TGG CTG TTT CTG GCT GTT ACT GTT GCT GAT GGC CTG ATT GTC |
GAPDH | Forward Reverse | CAC TCA CGG CAA ATT CAA CGG CAC GAC TCC ACG ACA TAC TCA GCA C |