Fig. 2

150 mg/kg PD suppresses Ly6G⁺ neutrophil and F4/80⁺ macrophage infiltration in intestinal inflammation. The images of immunohistochemical analysis for the (A) anti-Ly6G and (C) anti-F4/80 of colonic sections from healthy (Cont.) and DSS-exposed mice treated with or without PD (150 mg/kg and 300 mg/kg). Images were taken at 20X magnification (scale bar: 50 μm, shown in the upper right) and 40X magnification (scale bar: 20 μm). (B) Ly6G positive cells and (D) F4/80 positive cells were counted in three random inflamed mucosa, respectively, and presented as mean ± SEM, n = 5 mice. *p < 0.05, **p < 0.01, ***p < 0.001. PD, Pulsatilla decoction