Skip to main content
Fig. 6 | BMC Complementary Medicine and Therapies

Fig. 6

From: Harpagoside attenuates local bone Erosion and systemic osteoporosis in collagen-induced arthritis in mice

Fig. 6

Effect of harpagoside (HAR) on RANKL-induced osteoclast differentiation, bone resorption, and signal pathway in bone marrow macrophages (BMMs). A BMMs were cultured for 4 days in the presence of M-CSF (30 ng/mL) and RANKL (50 ng/mL) with HAR (0–100 μM). Then, the cells were fixed with 3.7% formalin, permeabilized with 0.1% Triton X-100, and stained with TRAP. Scale bar, 250 μm. TRAP-positive multi-nucleated osteoclasts (TRAP+ MNCs) with more than five nuclei were counted. B Mature osteoclasts were seeded on hydroxyapatite-coated plates and treated with the indicated concentrations of HAR for 24 h. The attached cells on the plates were removed and photographed under a light microscope. Pit areas were quantified using Image J. C-E BMMs were pretreated with HAR (100 μM) or vehicle (DMSO) for 1 h before RANKL (50 ng/mL) stimulation at the indicated times. The cell lysates were analyzed by western blotting with the indicated antibodies. F The mRNA expressions of TRAP, OSCAR, CTR, and Cathepsin K were analyzed by real-time RT-PCR. **p < 0.01 and ***p < 0.001 versus the vehicle

Back to article page