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Table 3 The IC50 µg/mL ± SEM of cytotoxicity against two cancer cell lines and one non-cancerous cell line of VLC isolated fractions, isolated pure compounds and ethanolic extract of D.cochinchinensis) and comparison with a standard chemotherapeutic drug (Vincristine sulfate) (n = 3)

From: Exploring in vitro anti-proliferative and anti-inflammatory activities of Prasachandaeng remedy, and its bioactive compounds

Sample

Code

%yield (w/w)

IC50 µg/mL ± SEM and Selective index (SI)

HepG2

KKU-M156

HaCat

Fraction 1 (hexane)

F1

0.19

NT

NT

NT

Fraction 2 (hexane: CHCl3, 1:1)

F2

2.31

 > 100****

 > 100****

 > 100****

Fraction 3 (CHCl3)

F3

14.57

43.14 ± 1.50**** (SI = 2.2)

42.26 ± 1.07**** (SI = 2.2)

92.96 ± 2.22****

Fraction 4 (CHCl3: MeOH, 1:1)

F4

78.65

51.95 ± 0.37**** (SI = 1.8)

61.51 ± 5.08**** (SI = 1.5)

95.01 ± 0.42****

Fraction 5 (MeOH)

F5

4.27

 > 100****

 > 100****

 > 100****

Loureirin B (1)

P1

0.10

20.02 ± 0.46**** (SI = 4.3)

21.26 ± 3.17*** (SI = 4.1)

86.12 ± 2.13****

4-Hydroxy-2,4’-dimethoxydihydrochalcone (2)

P2

0.12

20.71 ± 0.49**** (SI = 3.2)

33.21 ± 2.10**** (SI = 2.0)

66.00 ± 0.68****

Eucomol (3)

P3

0.15

25.76 ± 1.56**** (SI = 1.8)

7.12 ± 0.56**** (SI = 6.5)

46.55 ± 0.88****

EtOH of D.cochinchinensis

DC95

10.45

7.72 ± 1.87**** (SI = 5.2)

5.27 ± 5.01**** (SI = 7.7)

40.47 ± 0.39****

Vincristine sulfate

-

-

0.012 ± 0.0005 (SI = 0.00058)

0.0026 ± 0.001 (SI = 0.0026)

0.000007 ± 0.00

  1. NT mean not tested due to weight of extract less than 5 mg. SI Selective index calculated by IC50 of non-cancerous cells / IC50 of cancer cells. Data were presented as mean ± SEM and analyzed by one-way ANOVA and Dunnett’s multiple comparison tests. Significant different presented the ****p < 0.0001 compared with a standard drug (Vincristine sulfate) in corresponding cell line