cytochromes | Internal reference | Mobile phase | wavelength |
---|---|---|---|
CYP1A2 | 7-Hydroxycoumarin | Methanol: phosphate buffer (pH = 3.0, 50 mM) = 32:68 | UV 245 nm |
CYP2A6 | - | Acetonitrile: acetic acid (0.1%, v/v) = 35:65 | Fluo Ex/EM 340/456 nm |
CYP2B6 | Hydroxybupropion | Acetonitrile: water (v/v) = 20:80 for 2.5 min, 90–10 for 4 min | UV 268 nm |
CYP2C8 | - | Methanol: Water = 65:35 | UV 230 nm |
CYP2C9 | Coumarin | Acetonitrile (A): phosphate buffer (pH = 7.4, 100 mM, B) = 32:68, 0–9 min, 68% B-32% B | UV 280 nm |
CYP2C19 | Tolbutamide | Methanol: potassium phosphate (pH 7.0, 10 mM) = 30:70 | UV 204 nm |
CYP2D6 | - | Acetonitrile: phosphate buffer (pH = 3.0, 50 mM) = 25:75 | Fluo Ex/EM 235/310 nm |
CYP2E1 | Phenacetin | Acetonitrile: acetic acid (0.5%, v/v) = 22:78, 1–10 min, 78% B-40% B | UV 287 nm |
CYP3A4 | Corticosterone | Methanol: water = 50:40, 0–15 min, 48% B-30% B; 15–22 min, 30% B-20% B | UV 254 nm |