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Table 2 The detection conditions of HPLC for each CYP450 isoenzymes

From: Human liver microsomes study on the inhibitory effect of plantainoside D on the activity of cytochrome P450 activity

cytochromes

Internal reference

Mobile phase

wavelength

CYP1A2

7-Hydroxycoumarin

Methanol: phosphate buffer (pH = 3.0, 50 mM) = 32:68

UV 245 nm

CYP2A6

-

Acetonitrile: acetic acid (0.1%, v/v) = 35:65

Fluo Ex/EM 340/456 nm

CYP2B6

Hydroxybupropion

Acetonitrile: water (v/v) = 20:80 for 2.5 min, 90–10 for 4 min

UV 268 nm

CYP2C8

-

Methanol: Water = 65:35

UV 230 nm

CYP2C9

Coumarin

Acetonitrile (A): phosphate buffer (pH = 7.4, 100 mM, B) = 32:68, 0–9 min, 68% B-32% B

UV 280 nm

CYP2C19

Tolbutamide

Methanol: potassium phosphate (pH 7.0, 10 mM) = 30:70

UV 204 nm

CYP2D6

-

Acetonitrile: phosphate buffer (pH = 3.0, 50 mM) = 25:75

Fluo Ex/EM 235/310 nm

CYP2E1

Phenacetin

Acetonitrile: acetic acid (0.5%, v/v) = 22:78, 1–10 min, 78% B-40% B

UV 287 nm

CYP3A4

Corticosterone

Methanol: water = 50:40, 0–15 min, 48% B-30% B; 15–22 min, 30% B-20% B

UV 254 nm