Fig. 5

TIGAR expression is up-regulated in human GPL samples than in normal mucosa samples, and GRg3 can effectively reduce the TIGAR expression level in MNNG-induced GPL rats. A Expression of TP53-induced glycolysis and apoptosis regulator (TIGAR) in gastric cancer from data retrieved from the Cancer Genome Atlas database. B Results of enrichment analysis of a TIGAR gene set. C Determination of TIGAR in human gastric mucosa samples from various groups by an immunohistochemistry (IHC) assay (200× and 400× magnification). D Determination of TIGAR in rat gastric mucosa samples from various groups by an IHC assay (400× magnification). E TIGAR protein expression determined from cumulative mean optical density. The results are expressed as the mean ± SEM (n = 10). F TIGAR protein expression in human gastric mucosa determined from the immunoreactivity score. The results are expressed as the mean ± SEM (n = 10). G Determination of TIGAR in rat gastric mucosa from various groups by WB. H TIGAR protein expression determined by western blotting (WB). The results are expressed as the mean ± SEM (n = 3). #P < 0.05, ##P < 0.01 vs normal group; *P < 0.05, **P < 0.01 vs model group; ▲P < 0.05, ▲▲P < 0.01 vs folic acid group