Reversal of cisplatin triggered neurotoxicity by Acacia hydaspica ethyl acetate fraction via regulating brain acetylcholinesterase activity, DNA damage, and pro-inflammatory cytokines in the rodent model

Table 4 Effect of CisPT and different treatments of AHE on brain tissue protein, oxidative stress markers and lipid peroxidation

Treatment (mg/kg)	Protein (µg/mg Tissue)	H₂O₂ (nM/min/mg Tissue)	Nitrite (content µM/ml)	TBAR (nM/min/mg protein)
Control	2.330 ± 0.046⁺⁺⁺	1.743 ± 0.039⁺⁺⁺	47.84 ± 1.156⁺⁺⁺	5.026 ± 0.301⁺⁺⁺
CisPT	0.801 ± 0.125^***	4.946 ± 0.051^***	85.00 ± 1.055^***	11.92 ± 0.321^***
AHE alone	2.370 ± 0.019⁺⁺⁺	1.707 ± 0.013⁺⁺⁺	45.46 ± 1.302⁺⁺⁺	5.007 ± 0.183⁺⁺⁺
AHE (200) + CisPT	1.81 ± 0.045 ^***,++	3.381 ± 0.036^***,+++	66.73 ± 1.620^***,+++	8.643 ± 0.279^***,+++
AHE (400) + CisPT	2.298 ± 0.076^+++,##	2.04 ± 0.047^**,+++,###	50.84 ± 1.051^+++,###	6.119 ± 0.077^+++,###
Sily + CisPT	2.285 ± 0.039⁺⁺⁺	2.109 ± 0.07^**,+++	49.13 ± 1.075⁺⁺⁺	6.171 ± 0.191^*,###

Data expressed as mean ± SEM (n = 8). Astericks ^{*, **, ***} indicted significant difference of final body weight of group Vs. Control group at p < 0.05, p < 0.001 and p < 0.0001 respectively, ^{++, +++} indicated significant difference of final body weight of group Vs. CisPT-treated group at p < 0.001 and p < 0.0001 respectively, ^{##, ###} indicated significant difference of final body weight of AHE (200 mg/kg) + CisPT treated group Vs. AHE (400 mg/kg) + CisPT treated group at p < 0.001 and p < 0.0001 respectively. Non-significant difference (p > 0.05) was recorded between control and AHE alone treated group in all parameters. (One-way ANOVA followed by Tukey’s multiple comparison tests). Sily-Silymarin

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