Primer | GC% | Length (bp) | TM | Sequences (5′- > 3′) | PCR Product length |
---|---|---|---|---|---|
Cas9:F | 55 | 20 | 60.39 | ACATCTTCAATGGGACCGGC | 85 bp |
Cas9:R | 52.38 | 21 | 60.20 | TCTTTCTGCTCACCACCACAG | |
GAPDH:F | 50 | 20 | 59.96 | AAAGAGATGCTGAACGGGCA | 100 bp |
GAPDH:R | 47.62 | 21 | 59.79 | ACAAGGGAAACTTGTCCACGA | |
Bcl-2:F | 50 | 20 | 57.78 | GGAGGATTGTGGCCTTCTTT | 100 bp |
Bcl-2:R | 50 | 20 | 57.98 | GTCATCCACAGAGCGATGTT |