Table 2 Primers for Real-Time Quantitative PCR
Genes | Primers | Size (bp) | Annealing temperature (C) |
|---|---|---|---|
IL-4 | F: CTCACAGAGCAGAAGAACAC R: TGGTTGGCTTCCTTCACAG | 223 | 60 |
MAPK1 | F: GGAACAGCACCTCCACTATTT R: GCCACAATGTCTGCGTATCT | 226 | 60 |
NF-kβ | F: GCTGAGTCCTGCTCCTTC R: GTCTATTTGCTGCCTTGTGG | 208 | 59 |
TNF-α | F: GAGCCAGCTCCCTCTATTTATG R: CTACATGGGAACAGCCTATTGT | 189 | 60 |
PPAR-γ | F: ATGACAGACCTCAGACAGATTG R: AATGTTGGCAGTGGCTCAG | 210 | 55 |
IL1-β | F: GATGGCTTATTACAGTGGCAATG R: AGTGGTGGTCGGAGATTCG | 137 | 56 |