Fig. 4

The thermal treatments effect on Ajwain nsLTP1 secondary structure. Collagen was used as a control in this experiment. A nsLTP1 CD spectra were collected at 20, 40, 60, 80, 95 °C, and after cooling down to 20 °C. There were minimal changes in the secondary structure at 95 °C; however, it is back to the original upon cooling it to 20 °C. B collagen was denatured as the temperature increased to 40 °C and remained denatured even after cooling it down to 20 °C