Fig. 4
Effects of STPE on NF-κB translocation and I-κB𝛼 expression in UVB-induced L929 cells. Western blot analysis showing the effect of STPE treatment on the expression of p65 and p-p65 in nuclear extracts, p65 in cytoplasmic extract, and I-κB𝛼 in the whole cell. Cytosolic proteins were normalized with SDHA and nuclear proteins were normalized with laminin B. The cells were grown in 6-well cell culture plates and treated with various concentrations of STPE for 24 h before UVB (20 mJ/cm2) treatment. After incubation for 24 h, cell lysates were collected and subjected to western blot analysis using antibodies specific for p65, p-p65, and I-κB𝛼. Intensity quantification of protein bands were analyzed by Quantity One software (Bio-Rad). Bars represent means ± SEM of three samples per group. The data were analyzed by one-way ANOVA followed by LSD post-hoc test. Means with “*” differ significantly (p < 0.05)