Fig. 5
Assessment of S. officinalis impact on translational protein biomarkers using the BioMAP Diversity PLUS Panel. Plot showing the biomarker profile of the S. officinalis extract. The X-axis shows the quantitative protein-based biomarker readouts across the 12 cell-based disease models (see Methods section for a brief description of each cell system), with log-transformed ratio of the biomarker readouts for the S. officinalis-treated sample (n = 1) over vehicle controls (n ≥ 6) presented on the Y-axis at concentrations of 2.5 μg/ml (green), 5 μg/ml (yellow), 10 μg/ml (orange), and 20 μg/ml (red). The grey region around the Y-axis represents the 95% significance envelope generated from historical vehicle controls. Biomarker activities are annotated in the plot when 2 or more consecutive concentrations change in the same direction relative to vehicle controls, are outside of the significance envelope, and have at least one concentration with an effect size > 20% (|log10 ratio| > 0.1). Antiproliferative effects are indicated by a thick grey arrow above the X-axis. A full list of biomarkers assessed in order for each of the 12 cell-based disease models is available in Additional file 2