Fig. 3

Relative mRNA expression of IL-1β, IL-6 and IL-8 after TNFα pre-treatment of T24 and comparison of the effects of lavender oils and linalool standard with ACHP treatment. The mRNA expression levels were determined with Real-time PCR using SYBR Green protocol. The relative expression of controls was regarded as 1. β-actin was utilized for normalization. A T24 cells were pre-treated with TNFα for 24 h then with ACHP, linalool and lavender EOs beginning of flowering and end of flowering for 6 h. B T24 cells were pre-treated with TNFα for 6 h then with ACHP, linalool and lavender EOs beginning of flowering and end of flowering for 24 h. C T24 cells were pre-treated with TNFα for 24 h then with ACHP, linalool and lavender EOs beginning of flowering and end of flowering for 24 h. DMSO treatment was used as a control of the treated cells. The experiments were repeated three times (n = 3) in triplicates. Asterisks indicate p < 0.05 compared to the control. Cross marks p < 0.05 compared to the ACHP treatment. Double cross shows p < 0.05 compared to the linalool treatment. Number sign indicates p < 0.05 compared to the beginning of flowering