Fig. 4

The enhancing effect of high glucose on the expression of Caspase-3 was mitigated by diosmin intervention. A Caspase-3 expression was measured using immunofluorescence assay, magnification × 200 (scale bar =50 μm), × 400 (scale bar =25 μm), respectively. B Western blot assay for detection the protein expression of cleaved caspase-3 and caspase-3. C Statistical analysis for the semi - quantitative of immunofluorescence assay and Western blot assay. The results were expressed as the mean ± standard deviation (SD) of three independent experiments. Compared with the control group, ** p < 0.01. Compared with the high glucose group, ## p < 0.01