Fig. 2

Diosmin restrained the high glucose-mediated apoptosis in HK-2 cells. Effect of diosmin on high glucose-induced apoptosis was determined with Annexin V-FITC/propidium iodide (PI) kit by flow cytometry. A. Flow cytometry results with Annexin V-FITC/PI staining. B. The ratio of apoptosis among different experiment groups. Apoptosis ratio was early apoptosis percentage plus late apoptosis percentage. Control: Cells were subjected to 5 mmol/L normal glucose in DMEM for 48 h; Diosmin: Cells were subjected to 5 mmol/L normal glucose in DMEM for 48 h and then treated with 10 μg/mL diosmin for another 48 h; High glucose: Cells were exposed to 30 mmol/L high glucose in DMEM for 48 h; High glucose + diosmin: Cells were stimulated with 30 mmol/L high glucose for 48 h and reacted with 10 μg/mL diosmin for another 48 h. The results were expressed as the mean ± standard deviation (SD) of three independent experiments. Compared with the control group, ** p < 0.01. Compared with the high glucose group, ## p < 0.01