Fig. 9

Effect of hispidulin on the protein expression level of VEGF (VEGFA) (A) and phosphorylation of Akt (B) and ERK1/2 (C) under high glucose (HG, 30 mM) condition. Normal glucose (NG,5 mM) was regarded as control group and HG was model group. In order to save and recycle antibodies and save time, the membrane was clipped into bands of corresponding molecular weight of proteins prior to hybridization with antibodies and the information was shown in the Supplementary Fig. S1. Data were shown as mean ± SD (n = 3) and analyzed by one-way ANOVA and followed by Dunnett’ s multiple comparison test. ^*P < 0.05, ^**P < 0.001 and ^***P < 0.001 versus HG group