Fig. 5
From: Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A
Tan-I inhibits fibrotic changes through INHBA. A NRK-49F cells were transfected with nonsense control (NC) or INHBA siRNA (siINHBA). After 24 h, cell lysates were collected and Western blot analysis was performed to measure the expression of INHBA, FN and Snail. Data represent mean ± SD. #p < 0.05 vs NC. ##p < 0.01 vs NC. ###p < 0.001 vs NC. N = 9 in each experimental group and one representative result of at least three independent experiments is shown. B NRF-49F cells were transfected with NC or siINHBA. On the second day, cells were treated 50 μM of Tan-I for another 24 h. Cell lysates were collected and Western blot analysis was performed to measure the expression of INHBA, FN and Snail. Data represents mean ± SD. ##p < 0.01 vs NC/DMSO. NS represents not significant. N = 9 in each experimental group and one representative result of at least three independent experiments is shown. Full-length blots are presented in Supplementary Fig. 5