Fig. 3

Tan-I ameliorates renal fibrosis in AAN mice. A Study design. Wide type C57 mice received two times peritoneally injection of aristolochic acid I (AAI) to induce aristolochic acid nephropathy (AAN). Mice were treated with Tan-I for two weeks from day14 and were sacrificed at day 28. B Renal fibrosis was assessed by Masson’s trichrome staining and then quantified. Bars = 100 μm. C The expression of FN, α-SMA, Snail1 and pSmad3 were analyzed by Western blotting and then quantified. N = 8 in each experimental group and one representative of at least three independent experiments is shown. Data represents mean ± SD. *p < 0.05 vs normal saline (NS)/DMSO. **p < 0.01 vs NS/DMSO. ***p < 0.001 vs NS/DMSO #p < 0.05 vs AAN/DMSO. ##p < 0.01 vs AAN/DMSO. ###p < 0.001 vs AAN/DMSO. Full-length blots are presented in Supplementary Fig. 3