Fig. 4

Analysis of FGF, MAPK/ERK, SREBP 1 and 2 signaling pathways in CDDP-treated embryos. Expression levels were quantified using RT–qPCR and normalized against β-actin. Data are represented by relative fold changes ± SEM with respect to control embryos (arbitrarily set to 1). A Relative mRNA expression levels of Fgf1a, Fgf2, Fgf4, Fgf10a and Fgf11b, as well as the receptors Fgfr1a and Fgfr2. None were statistically changed. B The relative mRNA expression of bRaf, cplα2, mknk2b, JUN, Egf and Btc was also detected. Only JUN expression was significantly upregulated. C Relative mRNA expression levels of the SREBP 1 and 2 signaling pathways showed that Srebp2 might be activated and that as a downstream factor, Fasn expression was elevated. N = 6, and the data are presented as the mean ± SEM