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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Proangiogenesis effects of compound danshen dripping pills in zebrafish

Fig. 3

Endothelial layer thickening and ectopic growth of optical vessels in the CDDP treatment group. A-E Different treatments with CDDP led to thickening of the endothelial layer in the optical arteries of Tg(fli1:EGFP) larvae. Scale bar, 50 μm. In the 1.2 mg/mL CDDP treatment group, ectopic sprouts F and tumor-like growth G could be observed. H Endothelial layer thickness (T) was calculated by subtracting inner diameter (Di) from outer diameter (Do) using the formula T = (Do—Di)/2. I Changes in the thickness of the endothelial layer were quantified and are presented as the mean ± SEM. CDDP promoted thickening of the endothelial layer, although only the 1.2 mg/mL group showed statistical significance. N = 15 for each group, and the experiment was replicated 4 times. J Twenty-four hpf Tg(fli1: EGFP) embryos treated with or without CDDP (1.2 mg/mL) for 48 h. The mRNA extracted from 20 embryos from the same group was pooled, and the expressions of VEGF receptors (Flt1, Kdrl and Flt4) and VEGF (Vegfaa) were analyzed by RT–qPCR. Both VEGF receptor 2 (Vegfaa) and Kdrl were significantly upregulated in the CDDP treatment group. The relative expression level of each gene was normalized to the fold change compared with the control group. N = 6, and the data are presented as the mean ± SEM

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